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We systematically study the bulk and finite size effects on the band structure of prototypical line node materials using density functional theory based computations.
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The aspirated lymph node material is sent for acid-fast stain (which is frequently positive), TB culture, and histology.
Adipose tissue samples, isolated while strictly avoiding lymph node material, were fixed in 10% formalin, embedded in paraffin, and cut in 5 µm sections.
The sentinel node material was retained on ice and thereafter in AIM V® media (Invitrogen) in all subsequent steps.
Aspirated lymph node material was expressed on to a glass slide with 3 ml of syringe air.
Tissue cores with normal lung or lymph node material were used as negative controls in the TMA slides.
Following surgical excision, biopsied lymph node material was bisected and DNA was extracted from a representative region of tissue as described below.
The viral, fungal and bacterial studies, as well as acid-fast testing on the biopsied lymph node material were all negative.
Approximately 1 cm3 portions of lesioned lymph node material (study 2) were placed into sterile universals containing buffered formalin for routine histological examination.
Histological analysis and immunophenotyping of biopsied lymph node material was carried out using routine processing methods as previously described (Thomas et al, 2001), with the exception that tissue sections were immunolabelled using an indirect immunoperoxidase technique.
As ISA table defines an experimental workflow process as a set of nodes (Materials or Data), the leftmost node is a material node and the rightmost node is a data node, in line with OBI assay declaration specifying inputs and outputs.
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