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Due to the high hydrophilicity of both compounds, almost no unspecific uptake in non-target tissue was observed.
Thus, based on the abovementioned characteristics of 18F-MK-9470, we expect no unspecific binding of this tracer in bone metastases.
The PSMA-specific HBED-CC dimer 68Ga]7 is cleared even faster than the respective monomer 68Ga]6 and showed nearly no unspecific liver uptake.
The BAM antibody was marked with Cy3 fluorochrome and the unspecific mouse IgG antibody with Cy5 to assure that the BAM antibody was attached to cantilevers B and C and no unspecific antibody was attached.
The limit of detection (LOD) was calculated to be lower than 30 copies (3 × SD, SD was the standard deviation of the lowest Ct detected by qPCR), and no unspecific amplification was found in negative controls.
Consequently, assuming that only non-CD44v6-mediated uptake was measured, our data indicate that there was no unspecific accumulation of 125I-Fab or 111In-Fab in any normal tissue.
Similar(23)
Blockade of the endogenous NO generation with an unspecific NO synthase inhibitor was successful in alleviating migraine pain [15], while selective inhibitors of the inducible NO synthase seemed to be ineffective in reducing or preventing migraine pain [16, 17].
Our aim was to study the central effect of an unspecific NO synthase inhibitor (L-NAME) on the mean arterial pressure (MAP), plasma nitrate levels (pNO), plasma arginine vasopressin concentration (pAVP) and hypothalamic arginine vasopressin mRNA content during polymicrobial sepsis induced by CLP.
As shown in Figure 3A, CKα was detected with up to 20,000-fold dilution, and there was no apparent unspecific detection of other proteins including human CKβ, EK1 and EK2, or other unknown human choline or ethanolamine kinases.
The electrophoresis results of these PCR amplicons indicate that no other unspecific amplification occurred.
No significant unspecific expression was detected in other chondrogenic or non-chondrogenic tissues except some transient, probably unspecific X-gal reaction in the prenatal epidermis and hair papillae.
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