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The full list of identified proteins is given in Supplementary file 1. Strains used in (A ) were AM7509 SGO1-SZZ TAPP), AM1176 (no tag), AM8226 (proteaSGO1-SZZ TAP, SGO1-SZZ(TAM1176no AM8184 (protagse-deficient, no tAM8226
A strain carrying just tetOs integrated at CEN4 but otherwise wild type was used as a no tag control (AM11060).
Tethering of Sgo1-tetR-GFP, Sgo1-100-tetR-GFP, or Sgo1-3A-tetR-GFP, all significantly increased Brn1-6HA levels at CEN4 compared to a no tag control.
The Excel file submitted with the original manuscript was unsorted and did not take the contaminant proteins that also co-purify with the "no tag" control into account.
(D ) Strains AM1176 (no tag), AM6006 (SCC2-6HIS-3FLAM1530715307 (SCC2-6HIS-3FLandSCC4) AM178827882 (SCC2-6HIS-3FLAG scc4m7) or Strains AM1176 (no tag), AM1145 (SCC1-6HAM155405540 (SCC1-6HandCC4) AM178857885 (SCC1-6HA scc4m7) were arrested in metaphase of mitosis following treatment with nocodazole and benomyl (to depolymerize microtubules).
All tagged animals were correctly identified on recapture and no tag loss was evident from image or live comparison.
Similar(6)
The no-tag control is also shown.
The no-tag intact Mis17 was not detected by the anti-FLAG antibody.
Relative to the no-tag control strain, Nhp10 binding was significantly enriched at RPS14B, RPS5 and RPS13.
Cut14 associated with ssa1+ and hsp90+ genes during G2, but the association was comparable to the no-tag control during S phase (Fig. 5D).
When Sir3 was tethered to tlc1Δ48 (lanes 16 and 17), telomeres remained 107-bp shorter than the wild-type no-tag control.
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CEO of Professional Science Editing for Scientists @ prosciediting.com