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MCF-7WTR cells exhibited a ≈five-fold increase in cAMP accumulation in response to PTHRP1 34, whereas MCF-7VEC cells showed little or no responsiveness to PTHRP1 34.
As for the other members of the Group IV of rice CPKs, no expression could be detected in the rice roots for Oscpk31, whereas Oscpk30 exhibited a low expression but no responsiveness to AM inoculation.
However our data are consistent with those of Aleksic et al. [ 41], who noticed that several colorectal cancer lines showed no responsiveness to oxaliplatin, compared with cisplatin, when Notch signalling was blocked.
For the animal studies, we selected one donor with no responsiveness to IL-4 and one donor who responded to IL-4 with similar elevated hIgE levels as the AD patients.
Like DHN1, DHN2 was induced by cold, heat, embryogenesis and ABA; however, it exhibited no responsiveness to drought, E. necator infection, SA or MeJA, and was also expressed constitutively in vegetative tissues under normal growth conditions.
Only four cell lines showed no responsiveness to IFN by flow cytometry, and two of these were excluded from the study because they presented a mutation in β2 m gene [ 19, 14] and recovered IFN-γ inducibility after reconstitution of wild type β2 m gene (not shown).
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To evaluate arteriolar responsiveness to NO, the NO donor SNP was iontophoretically applied to individual arterioles in rats exposed to either saline or 0.25 mg ROFA.
As Heritage and Maynard (2006, p. 16) summarize 'this manifests itself in little or no patient responsiveness to clinicians' diagnostic statements'.
These mice have reduced vascular reactivity to NO through a reduced responsiveness to cGMP elevation.
Treatment with leptin reduced food intake in exercised rats that were pretreated with vehicle, although no increase in responsiveness to leptin-induced anorexia after pretreatment with anti-IL6 antibody, AICAR or Rapamycin was detected.
Most important, with respect to IL-4 induction, there was no lack in responsiveness to IL-7, to IL-4 or to their combination in RA patients compared with in healthy controls.
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