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Further, the TF might disappear ("No TF"); an orthologous TF might be present, but the regulated gene lost ("No regulated gene"), and, finally, the TFBS might be absent with both TF and gene being conserved ("No TFBS").
We also found that binding sites of the NO regulated transcription factor TGA1 [ 75, 76] were overrepresented in one hour leaves.
MAdCAM-1 expression was also reduced by N-acetylcysteine and by two NO donors (SperNO, DETANO) suggesting that hepatic endothelial MAdCAM-1 is oxidant and NO regulated.
Importantly, for cell cycle genes, NO and Bt2cAMP consistently had the same directional effect on transcripts, although NO regulated more genes overall.
NO regulated 110 transcripts that annotated disproportionately to the cell cycle and cell proliferation (47/110, 43%) and more frequently than expected contained AU-rich, post-transcriptional regulatory elements (ARE).
There is no regulated mechanism of iron excretion, and thus the absorption of iron must be tightly regulated to maintain iron balance.
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Sixteen NO-regulated genes contain apparent E2F sites (Table 3A).
In the current investigation, ARE- containing genes including IL-8 and p21 were over-represented among NO-regulated genes.
Furthermore, a recent study from the Marletta lab describes the important higher-order interactions in sGC, elucidating the mechanism of NO-regulated stimulation of activity.
Recent studies have indeed found multiple NO-regulated pathways within C. reinhardtii, including copper stress response, hypoxia, and ammonium and nitrate transport.
Further, NO-regulated transcripts had ARE (post-transcriptional regulatory sites) in their 3' UTR and were p38 MAPK responsive more frequently than expected.
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