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No reconstitution was observed when murine cells from embryoid bodies undergoing random differentiation were transplanted.
On the other hand, Reitano and colleagues showed no reconstitution in pDC-mediated production of IFN-α in HIV-1 patients receiving anti-retroviral therapy [31].
Also, among control mice showing no reconstitution with human T cells in blood (n = 3) all lacked human T cells in spleen (data not shown).
In contrast, when 30 million cultured cells depleted of CD34+ cells+ cells were injected into sub-lethally irradiated adult mice, no reconstitution was detected in the peripheral blood (data not shown).
Reconstitution was observed in 10 denuded mice out of 38 mice transplanted with GFP-E14 mES cells, while no reconstitution was observed in denuded mice transplanted with either GFP-E14-EBs at day 7 or at day 0 (Figure 4A, 4B).
These are ready-to-use control liquids, intended as a control for urinalysis reagent strips, requiring no reconstitution or dilution.
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Consistent with this prediction, we observed no detectable reconstitution of NAD degradation and TRPM2 dependent Ca2+ transients with the dZF1 mutant (Figure 4B, left and right panels).
No immune reconstitution was evident at 174 days post-transplantation.
One boy with IL7Rα-Def SCID who received his first two transplants from his mother had no immune reconstitution at 408 days post-transplantation.
The CD34− compartment of the Sca-1lo cells contained virtually all expression of the erythroid TF Gata1 and had accelerated erythroid differentiation but no culture reconstitution potential, while CD34+ cells were multipotent.
For the combination of Syn-VC anDJ-1-GFPNPno no fluorophore reconstitution was observed, indicating that the interaction between DJ-1 and aSyn can only be detected when the tags are present in a specific orientation, as previously observed for other proteins in BiFC experiments.
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