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This yielded summarized read counts for 36,229 genes annotated by Ensembl 59. 12,632 of these genes had no reads across all lanes and were not considered further.
Well-established known miRNAs tend to show high numbers of reads for the miR and miR* sequences, and very small numbers or no reads elsewhere.
"Socialism yes, Chayo, no" reads some of the graffiti in Managua.
As expected, no reads mapped to the centromere.
Table 3 shows the number of uncovered bases to which no reads were aligned in each data set.
Preliminary sequence data from this mixture included no reads from the halophilic archaeon, Halobacterium sp. NRC-1.
16/25 were identified as regions with no reads, 6/25 were identified as nucleotide substitution calls, and 3/25 had both.
As with single nucleotide deletions, insertion sites will nearly always generate either no reads or a mutant read in the vicinity of the insertion (Figure 5C).
To gain a better understanding of systematic biases of each NGS platform, we analyzed the sequence composition of uncovered bases to which no reads were aligned.
Another 7,414 genes had no reads for at least one B6 and one D2 sample and were considered to be unreliable.
Bases can have no coverage because no reads mapped to their location, or because reads cannot be mapped to their location.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com