Immunostained cells were counted by the optical fractionator method (×40 objective, counting frame 50 × 50 µm).
Total epi-tumoural lymphatic vessel density (LVD) was calculated using a × 40 objective, counting every lymphatic within a 350 μm border around the tumour edge.
By any objective count, the number of genuinely innocent civilians killed by the Israeli air force in Gaza is lower than the collateral deaths caused by any nation in a comparable situation.
For many people, winning by a small percentage of votes is not as significant as it would be in stable democracies where precise and objective counting of votes has been routine for many generations.
It turns qualities into quantities, or collapses subjective variations and "motives", which may be incompatible, into objective counts.
Subjectively reported cough and objective counts of the number of coughs and their duration were made together with a measurement of citric acid induced cough threshold [ 23].
As nearly the entire section of each of three non-adjacent sections per sample was analysed, and as transversally sectioned microvessels hit the intersection points randomly, the method allowed objective counts.
Slides were viewed using a 40× objective for counting and a 100× oil-immersion objective for nuclear volume measurement.
After 48 h of transfection, the transfection efficiency was estimated under a fluorescent microscope (Zeiss Axiovert 200) with a 20x objective, by counting the number of cells expressing the co-transfected pnlsGFP (100 ng/dish) [50].
LC3 spot count was quantified using Ideas software (Amnis), which contains a specialised objective spot counting feature.
