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The parameters used to produce the assembly were as follows: –K 25 (25-mer size), –L 100 (minimum contig length used for scaffolding), –e 2 (delete contigs with coverage less than or equal to 2), and –t 5 (output no more than five transcripts from each locus).
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This screen identified no more than three transcripts; XIST, and two unique noncoding nuclear enriched abundant transcripts (NEAT) RNAs strikingly located less than 70 kb apart on human chromosome 11: NEAT1, a noncoding RNA from the locus encoding for TncRNA, and NEAT2 (also known as MALAT-1).
From the category of our studied exons we have shown that, for genes that have no more than four transcripts, we classified their exons into four categories based on their occurrence in the transcripts.
If we assume that a probe can hybridize to a target sequence with up to 5 mismatches, then we estimate that 91% of the probes hybridize to a unique transcript and 99% to no more than two different transcripts, which are almost always encoded by paralogs that arose from the most recent WGD event.
For genes that have more than four transcripts, we have also classified their exons into four classes, especially with the aim to investigate the tendency of two extremes: G1 and G4 group.
Therefore, probes were categorized as "transcript-specific" if their exact hits (100% identity over 25 base pairs) were to one transcript only and "gene-specific" if their exact hits were to (possibly more than one) transcripts of a single gene only.
Notes: * If the gene has more than two transcripts, the two largest are listed.
We only kept clusters with more than two transcripts which contained at least one bear EST.
In our case, the experiment identified more than thousand transcripts differing in abundance between the strains.
If more than two transcripts fit, we selected the best-fit transcript pair by comparison among them.
The same pattern was also found in the down-regulated transcripts of the leaves; more than three hundred transcripts were down-regulated only in AC2 expressing plants.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com