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No matrix was added.
No matrix was used in the experiments.
For example, no matrix was detected in areas containing OVCAR5 cells (Figure 5I & J) while intense signals were detected for OVCAR10 cells (Figure 5K & L, see patches of red).
Each day of the validation, a method blank (no matrix) was prepared following the procedure described in " Sample treatment procedure" and analysed in order to check for any possible reagent or material cross-contamination.
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Transwell inserts (BD Biosciences) pre-coated with laminin, fibronectin, collagen IV, or control (no matrix) were used, and cells were permitted to invade for 48 hours.
We therefore expanded all TF-target links to all members of the corresponding TF (sub- family, for which no matrix isub- familyailable.
CDP choline, CTP and cytidine were expressed as relative concentrations (based on MS peak area ratio of analyte vs internal standard), because these are endogenous metabolites, and so no blank matrix was obtainable to use for standard curves.
Finally, it is found that chondrosarcoma cells spread well and synthesize extracellular matrix components only on the collagen ending films, whereas no cellular matrix was found for HA ending ones.
Although no single matrix was chosen for all genes, the preferred matrixes comprised a relatively small set.
For the separate association analyses of the three MCLUST groups, only the kinship matrix K but no P matrix was considered.
The sampling adequacy was confirmed by an anti-image correlation matrix, the factorability was proved by a Kaiser-Meyer-Olkin index that was >0.6, and the result of Bartlett's test of sphericity indicated no identity matrix was present.
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