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Discover Ludwig'no is required' is not correct and usable in written English
You would use it when you want to state that no action is necessary. For example, "No paperwork is required for this process."
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Thus, NO is required for root organogenesis (Pagnussat et al. 2002), the formation of adventitious roots (Pagnussat et al. 2003), lateral root development (Correa-Aragunde et al. 2004) and root hair formation (Lombardo et al. 2006).
These results therefore support the notion that a discrete inhibition of the respiratory chain as produced by NO is required to reduce oxidative rates and to allow glycogen or fat deposition (Figure 7B).
But whereas, in the absence of urate, 40 50 mM NO is required to reduce 1 mM cytochrome c, in the presence of urate only about 2.5 mM NO is required.
However, there is evidence that NO is required for LPS-induced MCP-1 or MRP1 expression [ 38].
In their seminal work, Correa-Aragunde et al. (2004) found that NO is required for LR formation in tomato plants.
Altogether, these results suggest that a decrease in macrophages, and the resultant reduction in NO, is required for the onset of labor.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com