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DNA from these parents was analysed by PCR using primers (5'-CCTGCGTTTGGAGATGACGAAATC-3' and 5'-CTTACATATAGAGCAGTGCGCTCACATG-3') that anneal to genomic sites flanking the insertion site producing a WT (no insertion) amplicon were discarded.
Virtual PCR was performed for each locus using the in silico function of BLAT (Kent 2002) to obtain the estimated PCR product size for the empty (no insertion) and the filled size (insertion present).
The results reveal 300 bp TCAST2c element inserted into both alleles in strains GA1 and GA2, in seven strains it is inserted in a single allele, whereas strain 43 has no insertion.
No insertion of lithium in NbO was observed.
No insertion complications or adverse clinical effects were noted during hospital stay [20].
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No insertion-containing regions were found with an |iHS| >2.5 in all eight populations.
After applying additional filters (≥10X coverage in all the individuals and no insertion-deletion polymorphisms at the SNP loci in any individual), this SNP set was refined down to a total of approximately 495k candidate SNPs (Table 1).
The current data have shown no insertion, deletion and nucleotide substitution in the siRNA target sequence, therefore demonstrated siM2 possessed good long term inhibition effect for influenza virus replication without the problem of siRNA resistant mutants.
There were no insertion/deletions or premature stop codons detected in DRB exon 2 in raccoons, suggesting it is functional.
Alignment of the seven genes sequences from 235 isolates showed no insertion/deletion (indel) in six genes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com