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The column was washed with lysis buffer until the A280 reached a minimum level where no further was observed (approximately 200 mL) and the PFTase enzyme was eluted using the above lysis buffer supplemented with 250 mM imidazole.
Similar(58)
No further explanation was given.
No further information was given.
No further action was taken.
No further information was forthcoming.
No further information was available.
No further meeting was planned.
This time, no further appeal was needed.
He said no further investigation was required.
Mr. Levine said no further treatment was necessary.
No further action was taken against Alty.
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