Sentence examples for no double peaks from inspiring English sources

Control, amplified products from target regions with no double peaks near the PAM sequence.

Direct sequencing of PCR products for 62 of these individuals generated a single sequence with no double peaks, which is identical to the full-length genomic fragment.

In all these cases no double peaks could be observed in the electropherograms; the presence of double infections therefore seemed unlikely.

All single-lineage sequences (n = 73, those containing no double peaks) of Plasmodium and Haemoproteus were input as homozygous sequences in the phase input.

Coding gene sequences were unambiguously translated into their amino acid sequence and no double peaks were observed in the chromatographs, suggesting sequences were of mitochondrial origin and not nuclear copies.

These mitochondrial sequences were assumed not to correspond to nuclear pseudogenes, as the mtDNA protein-coding genes considered did not include stop codons or frameshift mutations and no double peaks appeared in the corresponding chromatograms.

Unidirectional sequences were considered successful when high quality chromatograms (i.e. no double peak patterns and high fluorescence signal) were obtained for at least 200 bp.

For each primer pair, a preliminary real-time assay was performed to evaluate the amplification of non-specific products or primer dimer artefacts (no double peak in melt curve analysis).

To test this possibility, we manually examined the region around the most frequently cleaved base pair on each chromosome (e.g., Figure 2E,G,I,K) but detected no double peak pattern similar to that seen over centromeres.

For each pair of primers, a preliminary real-time assay was performed to check for amplification of non-specific products or primer-dimer artifacts (no double peak in melt-curve analysis), and slope values and the correlation coefficients (R2) were determined to assess the efficiency (E) of qRT-PCR.

Spectra were judged acceptable if the following criteria were met: FWHM of metabolites < 0.07 0.1 ppm, no unexplained features in the residuals, no doubled peaks or evidence for movement artifacts, symmetric lineshape, and no outer-volume ghosts or other artifacts present.