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However, when histological grade was taken into account, papillary ICC tumor did show significant differences compared to its adjacent non-tumor tissue D-NT, while well-differentiated tissue exhibited no differentiation with paired distal tissue.
Interestingly, a central population cluster was formed by Mexico City, Veracruz, the State of Mexico (Fig. 3), and Guanajuato, the latter displaying no differentiation with Mexico City.
These were tested for divergence from the null distribution of no differentiation with 10 000 permutations, as implemented in Arlequin.
In addition no differentiation with respect to the infectious focus was made in the study of Friedland et al. [ 10].
Port au Prince showed significant differentiation with four of the other six populations, while the La Poudriere population showed no differentiation with any of the other populations.
The degree of differentiation is scaled from 3 to 5 (grades 1 and 2 are no longer in wide use), with 3 to indicate tumors with glandular differentiation, 4 to indicate tumors with aglandular differentiation, and 5 to indicate no differentiation with cancer cells not organized into recognizable structures.
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Thus our findings do not support designation of "blue pike" as a subspecies or species, since it lacks reliable morphological characters and has no genetic differentiation, with no unique haplotypes or alleles.
Running STRUCTURE for k = 4, further confirmed notable genetic differentiation between rivers but no genetic differentiation with them.
Most studies of genetic population structure of amphibian show little or no genetic differentiation with moderate to high gene flow and dispersal rates at the local scale (<5-10 km), but stronger genetic differentiation at larger geographical scales (>15-20 >15-208, 12, 13].
Additionally, no differentiation was obtained with serial dilution and single cell plating in a 24- multi-well dish where single cell plating did pose a survival challenge (unpublished data).
In most studies, no differentiation was made with respect to underlying malignancies.
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