Prior to pupariation, mitotically active tissues of ball mutant larvae, including the brain, were severely reduced in size, whereas no defects were observed in postmitotic endoreduplicating tissue of larvae (supplementary material Fig. S4).
Likewise, Hr39 does not function in these neurons because no defects were observed in egg laying when Hr39 levels were reduced by driving Hr39 RNAi expression using ppkGal4.
In pkd2 (Obara et al., 2006; unpublished) and nphp6 (Sayer et al., 2006) knockdown, no defects were observed in cilia length and cilia motility in cloaca.
Isolated septal defects (but no outflow or aortic arch defects) were seen in three of 18 Citembryoslox;Mesp1Cre embryos and no defects were seen in the seven Cited2 +/flor;Mesp1Cre or nine Cited2 −/flox littermate control embryos studied (Supplementary material online, Figure S3 ).
No defects were observed in development, telomere length maintenance, or cell cycle regulation in tankyrase 1 or tankyrase 2 knockout mice.
No defects were seen in the 11 littermate control Cited2 +/flox; Sox2Cre or in eight Cited2 −/flox embryos.
Two heterozygous alterations in CDKN2A exon 1 were observed in melanoma tumor M13 one of which novel, whereas no defects were seen in the CDKN1A and CDKN2B genes.
But no defects were uncovered in any of the vehicles, and the rate of incidents was considered "unremarkable" in the context of the millions of cars on the road.
No defects were detected in the crystalline matrix of any of the observed nanowires which give evidence of their very good crystallinity.
No defects were seen in other tissues normally expressing CPA6, including the pectoral fin buds and somitic cells, which were analyzed following staining with DAPI (data not shown).
