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Further, microstructural studies reveal that a very thin layer of TGO was formed and obvious no coating failure or spallation was observed after thermal cycling test at 1150 °C for 500 cycles.

Interestingly, even the no coating control group showed a decrease in collagen II expression, as was observed with the protein coatings.

Cells placed on collagen I and aggrecan coatings showed significantly different gene expression profiles for collagen I over passage compared with the baseline passage and the no coating groups.

MCF10A and MCF10A CDH1-/ cells were seeded in six replicates at 2.0 × 10 cells per well in 96 well flat clear bottom plates (Greiner Bio-one) with different surface coatings: no coating for the uncoated, 2 μg/ml collagen (Sigma), 2 μg/ml fibronectin (BD Bioscience), 8 μg/ml vitronectin (Invitrogen), 8 μg/ml laminin (Invitrogen) and grown at 37°C, 5% CO2.

The study provides an insight into the effect of the concentration of reactants on the shell coating and shows that there exist nanoparticles which are completely coated, partially coated as well as particles with no coating too, with large fraction of partially coated nanoparticles.

Table 1 Contact angle measurements for different surface coatings Sample Contact angle No coating (quartz only) 45 ± 2° Un-doped ZnO 104.5 ± 5.9° Al-doped ZnO 3% 91.3 ± 1.0° Al-doped ZnO 7% 118.70 ± 0.63°.

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No coat rack.

No socks, no coat.

No coats most of em.

They wore no coats, carried no umbrellas.

Some have no coats, no shoes and no hat.

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