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However, no additional specimens could be retrieved from the historical American collections in the herbarium of Madrid.
It is possible that different tests for T cell differentiation and function (e.g., for proliferative or cytotoxic capabilities) would have revealed additional T cell characteristics, but no additional specimens were available for such analyses.
RNA was successfully extracted for all tissues with the exception of the S. sipylus PMG, for which the extraction failed and for which no additional specimens could be obtained.
All available samples were included in the study, and analyzed using CE-MS, no additional specimens that fit the criteria (2 years follow up, placebo or 300 mg Irbesartan daily) are available from the IRMA2 trial.
The obtained consensus sequence was used as reference to map other viral metagenomics data generated from the sheltered dog population; however, except for the aforementioned sample, KE528/2012, we found no additional specimens by this method to contain homologous viruses.
A fourth, cryptophthamic endemic, M. latens (Fig. 1) is currently known only from the formalin-fixed holotype and two paratypes, and despite considerable collecting efforts at the type locality of Bulu in the middle reaches of the LCR, no additional specimens have yet been obtained.
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Fluid-based specimens can be stored at ambient temperature for a longer period; therefore, this collection system offers the advantage that once the specimen has been collected, there is no need to collect additional specimens from the patient to perform a second Papanicolaou test or conduct further investigations.
However, because this exclusion might have introduced bias, we subsequently repeated the analysis including these 9 additional specimens and found no differences in the statistical significance of the models.
No additional biological specimens were taken for the purpose of this study.
three additional specimens were found in 2004. in addition to records from roadkilled specimens at three further sites.
DNA samples were obtained mostly from museum specimens and additional specimens collected during field work in 2009 in New Zealand (Landcare Research global concession permit no. CA5160-OTH) and Tasmania (Department of Primary Industries and Water permit no. FA08264).
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