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Experiments with no added cellobiose and without BG were also conducted.
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In the case where Novozyme 188 was not added, cellobiose accumulated in the medium up to 1.6 g/L.
In the case of experiments without added cellobiose, the reactions were provided with N188BG to prevent the inhibition of the CBH by the cellobiose released during hydrolysis.
C-amorphous cellulose (0.5 g l-1) was incubated (with shaking at 700 rpm) with EG in 50 mM sodium acetate buffer, pH 5.0, containing BSA (0.1 g l-1) in the presence and absence of added cellobiose.
"No," added Switzer.
EGI activity was measured by adding cellobiose to inhibit CBHI and glucose to inhibit β-glucosidase.
To match experimental work conducted as part of this study on cellobiose, it was necessary to add cellobiose degradation reactions to the draft model based upon the annotation and experimental evidence (EC 3.2.1.21, Tfu_0937).
No added sugar.
The other had no added radial bundles.
No added factors (negative control).
"No," Captain Watts added quickly.
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