Besides build-in support for MS /MS)- and H/C-NMR-type measurements by convenient short notations, specification of generic measurements is possible.
DSS was purchased from Cambridge Isotope Laboratories (CIL) (Andover, MA, USA); D2O and 5 mm NMR tubes type Norell 502 from Euriso-Top (Gif-sur-Yvette, France).
In addition glycan analysis is often performed not just using one method or technique but by utilizing several orthogonal methods including array-based ligand-binding assays, liquid chromatography (LC), CE, NMR, various types of MS such as mass profiling and tandem MS or hyphenated analytical methods such as GC MS or LC MS.
The system prepares a test solution by mixing a 15N-labeled protein solution and a solution of each candidate compound, loads it to a flow cell-type NMR probe, and recycles the protein after the data acquisition.
Magnets of this type (NMR-CUFF = Cut-open, Uniform, Force Free) are ideal for portable use and are eminently suited to investigate small or slender objects that are part of a larger or immobile whole, such as branches on a tree, growing fruit on a plant, or non-metallic tubing in industrial installations.
Thus the elements 'spectrum type = "NMR"' and 'reaction type = "reversible"' actually use different attribute types (spectrumType and reactionType), and it was for lexical convenience that both of these mapped onto the string 'type'type
The crystalline polymers, characterized by X-ray and 13C NMR, are a new type of stereoregular polynorbornene (PNB), having a 2,3-exo-disyndiotactic structure.
The same type of NMR spectra were collected at different pH values and the chemical shifts of haem methyls 12CH3I, 7CH3III and 12CH3IV were measured for different oxidation stages.
At the same time, a significantly larger fraction of non-bound F1(M158G/E159G/L161G) was detected by NMR compared with wild type (Supplementary Figure 10A, C), showing a reduction in affinity.
DOI: http://dx.doi.org/10.7554/eLife.05767.021 10.7554/eLiFigure67.022 Figure 5 figure supplement 2. Overlay of 2D NMR spectra for wild type K63-Ub2 and E64RP mutant at 50 µM with distal unit N-labeled.
