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Here, we describe the design and performance of a quasi-optical system enabling the use of a single 395 GHz gyrotron microwave source to simultaneously perform DNP experiments on two different 14.1 T (1H 600 MHz) NMR spectrometers: one configured for magic angle spinning (MAS) solid state NMR; the other configured for solution state NMR experiments.
C NMR spectra were recorded on a Bruker 400 Ultrashield (400 MHz) NMR spectrometers using dimethylsulfoxide (DMSO -d6 solvent.
The H NMR spectra were taken on a Bruker 400 Ultrashield (300/400 MHz) NMR spectrometers using dimethylsulfoxide (DMSO -d6 solvent anDMSO -d6ethylsolvent(TMS) andan intetramethylsilanechemical shifTMSn δ, ppm).
All NMR studies were performed on Bruker Advance III 500 or 600 MHz NMR spectrometers equipped with 5 mm single-axis z gradient inverse cryogenic probes.
The design and application of a versatile and inexpensive experimental extension to NMR spectrometers is described that allows to carry out highly reproducible PHIP experiments directly in the NMR sample tube, i.e. under PASADENA condition, followed by the detection of the NMR spectra of hyperpolarized products with high spectral resolution.
In modern NMR spectrometers, organic compounds are placed in magnetic fields ranging from about 1.4 to 18.0 teslas (T) and are irradiated with radio-frequency waves.
NMR spectrometers have a feature, called integration, which, when selected by the user, calculates the area under each peak and plots the result as a line that is displaced vertically at a peak by an amount proportional to the area under the peak.
These magnets can potentially be used in medium-resolution NMR spectrometers and high-field NMR spectrometers.
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All 1 H NMR spectra were recorded on 400 MHz Varian FT-NMR spectrometers.
1H- and 13C-NMR spectra were recorded in CDCl3 and CD3OD on Bruker Avance-NMR spectrometers.
1H-NMR and 13C-NMR spectra were recorded on Varian MR (300 or 400 MHz for 1H-NMR, 100 MHz for 13C-NMR) spectrometers at an ambient temperature.
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