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Absorbance was measured at 492 nm by a UV spectrophotometer.
The structures presented a polydisperse size from about 50 nm to even 700 800 nm by TEM and about 250 nm by DLS.
YW211.31 antibody has a binding affinity of about 2 nM by surface plasmon resonance (SPR) and 0.6 nM by Scatchard analysis.
Absorbance [optical density (OD)] was measured at 540 nm by using a microplate reader.
The emitted fluorescence signal was collected at 510 nm by a Regiga Exi camera.
Figure 2A shows the significant change of GSSG reduction (100 nM) by GSH forming.
MTT values were measured at 570 nm by a Biorad 3550 microplate reader.
The protein concentration was determined by dividing the absorbance at 280 nm by the extinction coefficient.
In liquid culture, cell density was determined at 600 nm by a Beckman Coulter spectrophotometer.
Absorbance was determined at 530 nm by spectrophotometry.
Samples were sectioned to 70 nm by an ultramicrotome (Leica).
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