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Soleus and EDL muscles were cut at mid-belly, transversely oriented, and frozen in isopentane liquid nitrogen "slurry" cooled to −158°C for subsequent immunohistochemical analysis.
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Then methanogenic bacteria metabolize these compounds into a combination of methane-rich gas and an odorless phosphorus- and nitrogen-laden slurry, which makes excellent fertilizer.
The vapors produced during the reduction were condensed in a cold trap immersed in liquid nitrogen and isopropanol slurry.
Fungal DNA extraction involved grinding mycelia in a pestle and mortar under liquid nitrogen, suspending the slurry in lysis buffer (200 mM Tris-HCl, pH 8.5, 250 mM NaCl, 25 mM EDTA, 0.5%SDS), extraction with phenol: chloroform: isoamyl alcohol, and ethanol precipitation [ 16].
But when the researchers mixed the cuprates with liquid nitrogen and placed the slurry between two electrodes, the cuprate grains clung to each other in a single ball.
Normally, rural households use the raw manure obtained from their animals as a form of plant fertilizer, but this has a lower organic nitrogen content than the slurry created by the biogas digestion process [5 7], which is odourless, and makes a better fertilizer.
Operator input of slurry total nitrogen content, determined by onboard hydrometer in the field, and target application rate in the control unit ensured that nitrogen was applied at a constant rate based on metering pump rotational speed as a function of machine forward speed.
Total ammoniacal nitrogen content in the slurry was reduced from 4.32 g N kg−1 to 3.13 and 1.92 g N kg−1 when dietary CP decreased from 20%to1616% and 12%, respectively (the corresponding figures for total nitrogen content were 5.48, 4.30 and 3.05 g N kg−1, respectively).
van der Stelt et al. (2008) found that reducing dietary crude protein level by 25%% of 200 g/kg of dry matter result in 21 and 66 % decrease of N-losses in faeces and urine respectively, while on the other hand 75%% increase of 108 g/kg crude protein led to 56%% increase in total Nitrogen loss in the slurries.
A comprehensive field study was conducted to determine the fate of nitrogen in pig (Sus scrofa) slurry applied to an acid tropical andic soil of Réunion with the aim of estimating drainage and nitrogen leaching below the root zone.
The present study was carried out to investigate how three different fibre sources, sugar beet pulp, soya bean hulls and pectin residue, in diets for growing pigs influenced the concentration of short-chain fatty acids (SCFA) in faeces, pH-value in faeces and slurry, excretion of nitrogen in urine and faeces and ammonia emission from slurry under dynamic conditions.
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