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The n-hexane:dichloromethane extract was concentrated to dryness, using firstly a rotary evaporator and then a gentle stream of nitrogen (heating and evaporating units).
As HuffPost noted in 2013, a couple of science professors from Emory University documented dunking some unlucky marshmallow chicks in liquid nitrogen, heating them up and even subjecting them to a vacuum.
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A nitrogen heat pretreatment enhances the stability and activity.
The solution was deaerated using nitrogen, heated to 73°C, and a deaerated solution of benzoyl peroxide (1.5 g) in styrene (37.5 mL) was then added.
The portion of this tube that extends out of the vacuum chamber is surrounded by an enclosure, through which nitrogen, heated to ~85 °C, flows.
Subcooled liquid nitrogen is produced for a commercial transportation container with a house-made atmospheric liquid nitrogen heat exchanger and then, having subooled degree of up to 19 K, it simulates the behavior of subcooled LNG in the lab-scale reliquefaction experiment.
Benchtop micro-centrifuge Nitrogen Heat-block 1.5 ml microfuge tubes, DNAse free.
To produce fresh inocula for the experiments, uredospores were taken out of the liquid nitrogen, heat shocked (40°C, 15 min) and used to inoculate 7-day-old seedlings of the susceptible cultivar Victo.
The following conditions were found to be optimal for the analysis of SkQ1: capillary voltage, 3.0 kV; source block temperature, 120°C; and desolvatation gas (nitrogen) heated to 450°C and delivered at a flow rate of 800 L h-; cone voltage, 55 V; cone Gas Flow rate, 50 L h-.
Various laboratory reactions that yield nitrogen include heating ammonium nitrite (NH4NO2) solutions, oxidation of ammonia by bromine water, and oxidation of ammonia by hot cupric oxide.
Differential scanning calorimetry (DSC) measurements were carried out with a DSC 204 F1—NETZSCH instruments under nitrogen flow (heating rate 10 °C min−1).
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