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Plant nitrogen accumulation was linearly correlated with both nodule P and Fe concentration, and with total plant nitrogen fixation rate as measured by acetylene reduction rate.
Surprisingly, nodule ureide concentration, which is generally assumed to be a positive index of nitrogen fixation rate, was negatively correlated with nodule P and Fe concentrations.
Therefore, total nitrogen fixation rate was also correlated with nodule P and Fe concentrations, but a higher linear correlation was obtained for Fe as compared to P concentration.
Although 50 MtN y-1 is a nontrivial amount, this is only a fraction of both the global natural nitrogen fixation rate of 110 MtN y-1 and the anthropogenic N (mainly from fossil fuel burning and fertilizer use) deposition rate of 140 MtN y-1 [33].
The nitrogen fixation rate per nodule and bacteroid is reduced as well.
The rate of nitrogen fixation of A. azollae is estimated at 1.1 t N/ha-year, which is significantly higher than the nitrogen fixation rate of the legumes, 0.4 t N/ha-year [ 23].
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This mixed cultivation increased the population growth and nitrogen fixation rates of Herbaspirillum when measured by acetylene reduction activity.
This study was designed to induce various concentrations of P and Fe in the nodule and to measure the resultant nitrogen accumulation and nitrogen fixation rates.
The nitrogen fixation rates by two different types of electrical discharges, namely lightning and coronae, were studied over a wide range in CO2 and N2 mixing ratios.
Adequate supplies of phosphorus (P) and iron (Fe) to legumes have been shown to be crucial in obtaining high nitrogen fixation rates and growth.
However, little attention has been given to documenting the response of nitrogen fixation rates resulting from concentrations of P and Fe that actually exist in nodules.
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