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The layers were produced using a d.c.-cathodic arc source with a titanium cathode and a nitrogen feed gas.
The complex nitrogen feed helped in increasing the cell density and volumetric activity by ∼9 and 18-fold respectively with respect to that achieved in minimal medium.
The X-ray sensitive CCD is at the left end of the system, to which is attached a liquid nitrogen feed line for cooling to -75deg C. The detector electronics are in the foreground on a cart with four power supplies.
For all the conditions investigated, reactor configuration performance was in the order PBMR-E>FBR>PBMR-O, which could be enhanced further for the membrane reactors by increasing the residence time of reactants over the catalyst bed by partition of the nitrogen feed.
For culture (b), specific nitrogen consumption rate decreased following the sigmoidal nitrogen feed flow, with a delay of 20 minutes.
This approach will thereby provide a means to implement the proposed stoichiometrically-balanced nitrogen feed (36%N-NH4+andN-NH4+and64%3-).
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Mineral nitrogen feeding increased yield but did not affect yield enhancement by the bacterial partner.
Two cultures were performed: one conducted with nitrogen exhaustion and another with nitrogen feeding in limited concentrations, during the production phase.
The model equations were extrapolated to fed-batch cultivation by incorporating the dilution terms due to fresh nutrient (carbon and nitrogen) feeding.
In both cultures 73%% of P 3HB-co-3HV) was P 3HB-co-3HVt at the culture with nitrogen feeding, during the P 3HB-co-3HVhase, productivity reached 1.24 gP(3HB-co-3HV).L−1.h−1 and qP(3HB-co-3HV) remain at the maximum level (0.13 gP(3HB-co-3HV).gXr−1.h−1) during more time.
It can be concluded that the system with external cell recycle and nitrogen feeding, during the production phase, is a good alternative to utilize agro-industrial wastes with moderate carbon concentration and to increase productivity of P 3HB-co-3HV).
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