Cells (100 mg) were homogenized in liquid nitrogen containing 0.1%% tricloroacetic acid (TCA) using a mortar and pestle and centrifuged at 11,600×g for 15 min at 4 °C.
As three different types of nitrogen sources, NaNO3 is a source of inorganic nitric nitrogen; corn steep liquor is that of organic nitrogen, and its main components include polypeptide and amino acid; yeast extract is also a source of organic nitrogen, containing protein, amino acids, trace vitamin nucleosides, and other nutrients.
Plasma is highly rich in nitrogen containing compounds such as amino acids.
O-H · · · N interactions are found between carboxylic acid and the nitrogen containing heterocyclic ring of the acridine.
In this study, ammonia and sulphur dioxide gases have been chosen with the objective of incorporating carboxylic acid, sulphur and nitrogen containing groups on the surface.
In many plant species, considerable amount of nitrate taken up by roots is transported to the leaf cells, where it undergoes reduction to ammonium and conversion to amino acids and other nitrogen containing molecules.
When cells face such a situation they continue to assimilate the carbon source but, being unable to synthesize nitrogen containing metabolites like amino and nucleic acids, arrest growth and convert the carbon source into storage metabolites, mainly glycogen and neutral lipids.
A number of nitrogen containing compound such as ammonia and amino acids have been proposed as candidate compounds for nitrogen exchange in ECM systems [ 26- 28].
Bio-oil contains hundreds of compounds including carboxylic acids, alcohols, aldehydes, ketones, phenols, guaiacols, syringols, carbohydrates, furans, alkenes, aromatics, nitrogen containing organic compounds, and miscellaneous oxygenates [ 25].
Indeed, an ammonium transporter (p27877) and other nitrogen containing compounds transporters (p20424, p768, p52619) were found to be upregulated in the presence of silicic acid in P. tricornutum.
N-comp.: Nitrogen containing compounds.
