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The total C (TC) and total N (TN) contents in the samples from O horizons and samples of particle-size fractions from the O/A horizon were determined after combustion in a Nitrogen Analyzer (NA1500 Series 2, Fisons, Italy).
Soil organic carbon was determined by Walkley and Black's rapid titration method (Allison 1973) and total nitrogen content of samples was measured by Pelican automatic nitrogen analyzer (Model KEL PLUS India).
The total nitrogen content was determined using a nitrogen analyzer (Rapid N III, Elementar Analysensysteme GmbH, Hanau, Germany) according to the Dumas method, and the crude protein was calculated as total N × 6.25.
Total nitrogen of each protein fraction was determined by the automatic Kjeldahl nitrogen analyzer (Kjeltec 2400 Analyzer Unit, Foss Tecator) and protein content was calculated using a conversion factor of 5.95, as shown in the equation below: Protein % = N % × 5.95 (1).
Total organic carbon (TOC) and total nitrogen in the extracts were measured simultaneously on a TOC VCPH/CPN Total Organic Carbon and Nitrogen Analyzer (Schimadzu, Kyoto, Japan).
Briefly, 14 half-carcasses were macerated (WolfKing macerator) and 250 g dried, homogenized and nitrogen content determined by a FlashEA1112 nitrogen analyzer (Thermo Scientific, UK).
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Coupling of mass spectrometers to CHN (carbon, hydrogen, nitrogen) analyzers, combined with advances in computers that permitted extensive automation, has opened up the field to a far wider range of practitioners as well as permitted a vast expansion in the speed of analysis that can be completed.
The surface areas of zeolites were determined by a Micrometrics ASAP 2010 nitrogen adsorption analyzer.
Specific surface area of the catalysts was obtained by the BET (Brunauer-Emmer-Teller) method using Thermo Finnigan Sorptomatic 1900 series nitrogen adsorption/desorption analyzer.
Exhaled breath was measured with a chemiluminescent nitrogen oxide analyzer (model 200A; API, San Diego, CA).
The simultaneous residual lung volume measurement system included a calibrated piston pump which was used to dispense a measured volume of oxygen into a rubber bag and a fast responding electronic nitrogen gas analyzer.
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