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High nitrification results in low ammonium concentration, low ammonium uptake rate, and high nitrate uptake rate in the euphotic zone.
This study was conducted to find a relationship between nitrate uptake rate (NUR) and carbonaceous oxygen uptake rate (OUR) and develop a simple method to predict applicable NUR for the design of various BNR processes.
The nitrate uptake rate was calculated by linear regression analysis according to Pinton et al. [ 38].
At root level of nitrate-induced plants, the nitrate uptake rate increased with the rise of the external anion concentration, showing a biphasic pattern with a typical saturation profile up to 0.33 mM nitrate and a linear kinetic at higher anion concentrations.
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Subsequently nitrate uptake rates were calculated.
In contrast, expression of some nitrate transporter genes and nitrate uptake rates were strongly correlated.
Data presented here confirm that also in cucumber roots higher level of nitrate uptake rates are related to an increase in PM H+-ATPase activity and quantity.
During nitrogen limitation, one set of nitrate transporters (nrtBCD, MAE18880, 18890, 18900) was upregulated, correlating with nitrate uptake rates (Fig. 6a, b), while another set (nrtBCD, MAE14790, 14780, 14770) was downregulated (Fig. 6c).
During N starvation, expression of one nitrate transport gene set (nrtBCD, MAE18880, 18890, 18900) and uptake rates of nitrate remained static whereas restoration of nitrate was accompanied by the expression of genes for this nitrate transport gene set increasing 3-fold and nitrate uptake rates increasing 6-fold.
Thereafter, a decline in net nitrate-uptake rate was observed.
When the nitrate-uptake was measured in leaf discs, an enhancement in nitrate-uptake rate was evident and became maximal after 9 to 12 h of treatment.
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