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The study focused primarily on acetate oxidation by nitrate reducing bacteria.
Additional substrate (nitrite) could be provided by nitrate reducing bacteria (NaRB) for NOB growth.
It can be assumed that nitrate reducing plant leaf provides nitrate reductase enzyme (protein).
The composition of nitrate reducing community has been explored from various habitats.
Moreover, Simplicispira members of the Simplicispira genus have been isolated from wastewater and identified in nitrate reducing and PHA degrading enrichments (Dawson et al. 2013).
Our results suggest that nitrite and nitrate fluctuations in an environment will have limited influence on the dominant nitrate reducing process when acetate is the electron donor.
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The benzene degradation pathways of the nitrate-reducing Azoarcus strains29 have not been investigated in details.
This suggests involvement of monooxygenase-mediated degradation under nitrate-reducing condition80.
Fu, Q. et al. A thermophilic Gram-negative nitrate-reducing bacterium, Calditerrivibrio nitroreducens, exhibiting electricity generation capability.
The nitrate-reducing facultatively anaerobic Dechloromonas26 may recruit enzymes of a yet unknown pathway for initial benzene activation27.
In summary, our metatranscriptomic study of a benzene-degrading nitrate-reducing continuous culture provides insights into benzene degradation mechanisms.
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