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After nine passages the virus was able to correctly replicate in Vero cells, virus titer achieved was 105.875 TCID50 (median tissue culture infective dose) ml−1.
Interestingly, these iNANOG cells showed a significantly improved survival ability and clonogenicity when cultured in the primed state, and they could grow in 2i plus LIF conditions for more than nine passages.
Using fractional factorial designs combined with the steepest ascent approach, we have developed a serum-free medium that could ex vivo expand MSCs over nine passages, resulting in at least 1000-fold incellses inumber number within 1-month.
Each reading test featured nine passages with a total of 122 items including multiple choice, a limited number of multiple-response multiple-choice items (selected response items in which test takers must select two or more correct options per item), and one categorization item.
Averroes used two Arabic translations; he preferred the translation by Isḥâq Ibn Ḥunayn, but he quoted the older one as alia translatio also in nine passages (Crawford 1953, pp. 46, 86, 284, 452, 469, 480, 514, 519, 526), going through the entire book.
The U87MG cells remained diploid after nine passages.
Similar(48)
Consider the following two passages.
BC: Two passages stuck with me.
He mentioned one or two passages.
There are at least three passages for three couples.
The two passages are similar in that each one a.
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