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A bit of chicken is laid on a plate, with a bone thrusting skywards and accompanied by nil-by-mouth quantities of veg.
It is the frequency of these "nil" aliquots that allow the average target quantity to be calculated using the equation: (14 Average target molecules per aliquot = -Ln(Nil/Total) where Nil and Total are the number of reactions that fail to produce amplicon DNA and the total number of reactions conducted, respectively [ 36].
Among the latter four NILs, Aroona- Glu-B3c possessed the lowest quantity of B-group LMW-GS proteins (Additional file 1: Figure S1), especially the protein with the largest molecular weight.
The only exception was Aroona- Glu-A3e which possessed the highest number of gliadins and the lowest quantity (none) of i-type LMW-GS among the Glu-A3 NILs.
As addressed further below, an effective estimate of target quantity can be achieved by averaging the N0 values produced by replicate amplifications, although this average must include nil reactions (i.e. 0 N), as illustrated in Figure 8B.
See nil.
Probably nil.
Almost nil.
Hairdressers nil..
Nil desperandum.
Qualifications: nil.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com