Fig. 3 Procedure of NIL experiments and simulations with the pressure and temperature.
After the pressures of 10 and 12.5 bar were reached, they were maintained for 1 s and given the name, 'constant pressure step,' in all NIL experiments.
For better clarification of the functions of qcd4-1 and qcd4-2, further studies are required to isolate a gene underlying QTL and to perform NIL experiments on different environments.
In the UC1041 +/- Yr36 NIL comparison experiments the same procedure was used to apply urediniospores, but whole leaves were inoculated.
In four of the five NIL validation experiments the Rialto introgression gave significant improvements in yield (5.5 ± 1.5%) and TGW (5.1 ± 1.6%), with morphometric measurements showing that the increased grain weight was predominantly a result of wider grains.
In a similar experiment, NILs segregating for the Pup1 locus (+Pup1: NILs 6-4, Y-4, 14-4; −Pup1: NILs 26-6, 28-10, and Nipponbare) were grown for 50 days in either P-deficient or P-fertilized soil, and root material was sampled and immediately shock-frozen in liquid N. Some genotype information on these NILs can be found in Heuer et al. (2009), Torabi et al. (2009), and Wissuwa et al. (2002).
Mesocotyl of CSSL-6 was significantly longer than that of NIL-1 in the experiment to detect interaction between 2 QTLs (Figure 5).
To evaluate the differences in grain length between the recurrent parent 93 11 and its three NILs, all materials including 93 11, NIL- qgl3, NIL- GS3 and NIL- GS3/ qgl3 were grown in the Jiangpu Experiment Station of Nanjing Agricultural University.
Actual devices were fabricated using the machine including a microfluidic PDMS Y-channel mixer, via the laser adaptor, and an etched nanotopographical array for cell culture experiments, using the NIL head.
One cycle difference in the RT-PCR experiments corresponds to the two-fold difference in gene transcripts between the NILs examined by the microarray experiment.
