The trio probably hopped the fence around the pool late at night, sliding into the water as most of the people at the Chapel Tower apartment complex were sleeping.
Dr. Philip Eliades, Dr. Allen Miraflor and Dr. Luke Olson awaiting for sign out of the morning slides.
(7) Slides represent the pre-transport disposition of strata and their reservoir quality (i.e., porosity and permeability) of the provenance region, whereas debrites reflect post-transport depositional texture and reservoir quality.
The following morning, slides were washed and incubated with donkey Dylight or Alexa Fluor secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.) for 2 h, then washed again before being coversliped using Fluoromount-G (Southern Biotech, 0100-01).
The next morning, slides were washed in PBS (3x, 5 min each), and incubated with a Cy3-conjugated affinity purified donkey anti-rabbit secondary antibody diluted 1∶500 in the same buffer as used for dilution primary antibody, for 2 hours at room temperature.
The next morning, slides were washed once with PBS + 0.25% Triton, then once with PBS.
On the next morning slides were washed three times with PBS.
The next morning, slides were incubated 30 min with the appropriate secondary antibody conjugated to biotin, at a dilution of 1 500 (rabbit anti-mouse or swine anti-rabbit; DAKO, Cambridgeshire, UK).
The next morning the slides were washed three times (5 min each time) with PBST (PBS-0.1% Triton X-100).
Figure 7: Sliding versus paraesophageal hiatal hernia.
