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Since the adaptors are not 5′ phosphorylated, the nick repair polymerase in the kit repairs subsequently the nick on one strand at each ligation site, in order to minimize adaptor-dimer formation.
One mechanism for producing a DSB in G1 is to expand a nick on one strand into a gap, allowing for formation of a "hairpin" on the ungapped strand.
Duncan Smith denied that the idea was dumped due to opposition from Clegg, insisting: "I am fully at one with Nick on this".
He had a nick on his chin, from shaving.
There was a shaving nick on his collar.
Nick, on the other side, wanted a larger kit with a higher yield.
He only got a nick on it - full face and it would have flown away.
How he built a new life – warning, this book contains yoga – has set me and Nick on a new path.
But if you asked Nick on the wrong day, Chris said, you might get a different answer.
Weir says a nick on the milling pattern of a modern putter face can throw hundreds of putts off line.
There was a large bloody nick on his throat, and a corresponding red blossom on his shirt collar.
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