DNA (100 ng 500 ng) was extracted from UCWBCs.
Post irradiation, the genomic DNA (10 ng) was extracted from the S. aureus cells using Extract ME DNA Bacteria purification kit (Blirt S.A). and treated with endonuclease III (New England, BioLabs).
Genomic DNA (400 500 ng) was extracted from frozen SV tissue of individual mice using a Tissue Blood Kit (QIAGEN) according to the manufacturer's protocol.
This DNA was quantified using Picogreen (Invitrogen, Life Technologies, Grand Island, NY, USA) and for each sample a final quantity of 250 ng was extracted and used for GoldenGate genotyping with VeraCode Technology (Illumina Inc., San Diego, CA, USA).
After the addition of internal standards (H6-SA, 300 ng), samples were extracted using a mixture of acetone and 50 mM citric acid (v/v = 7/3), and ethyl acetate.
A total of 50 150 ng genomic DNA was extracted from peripheral blood using a GFX genomic blood DNA purification kit (Amersham Biosciences, Piscataway, NJ, USA), and amplified using the 296 (5′-GTGGATAGAGCAGGAGGCT-3′), and 302 (5′-CCAAGAGCGCAGGTCCTCT-3′) primers.
In general, 2 to 5 ng of RNA were extracted from dissected normal epithelial ducts and stroma.
500 ng genomic test DNA was extracted from either peripheral blood or BM samples.
Generally, 20 to 100 ng of total RNA were extracted from microdissected samples and subjected to mRNA amplification prior to hybridization to Affymetrix microarrays.
10 ng of purified genomes were extracted from adult animals and used as templates.
