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Secondly, two RT replicates from the same RNA sample (150 ng) were compared.
Of note, no bias was observed in the GC content when different quantities of total RNA (300 and 100 ng) were compared for the RT step.
To determine whether quality data could be obtained from a broad range of input RNA, data obtained from amounts ranging from 25 ng to 800 ng were compared to those obtained at 200 ng.
To evaluate the genotype concordance, all the TaqMan assay results from serial dilutions of gDNA and MDA DNA (1 ng, 0.1 ng and 0.01 ng) were compared to genotypes previously confirmed by direct re-sequencing of the same samples (50 ng).
Similar(56)
AUC/dose NG was compared to AUC/dose IV using a paired T test.
The difference in methylation between retina and brain (ΔM) at T-DMRs identified within the high-input samples (250 ng) was compared to the corresponding ΔM of these same T-DMRs from the low-input samples (10, 25 and 50 ng; Figure 4A).
The kinetics properties of GSs, EG and NG electrodes were compared by AC impedance measurements.
To achieve this, the time 0 references from RUT C30 and NG 14 were compared using RNA-seq experiments (Additional file 6: Table S3).
Duplicate samples were compared using 25 ng and 50 ng of RNA as input.
Mice or ferrets were vaccinated intramuscularly with VLPs in a dose sparing experiment, based on HA concentration (3 μg–24 ng), and the immune responses were compared to responses elicited in animals vaccinated with recombinant HA (rHA) or inactivated whole influenza virions (WIV).
Samples from T0 (before lactose induction) from NG 14 and RUT C30 were compared by RNA-seq.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com