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Denatured DNA (200 ng) was transferred onto a nylon membrane (Roche) by using a Bio Dot apparatus (Bio-Rad) according to manufacturer's specifications.
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Typically 100 ng and 500 ng of plasmid DNA were transferred to each well of the 96-well plate and 24-well plates, respectively.
A volume containing 50 ng of protein was transferred to a 1.5 ml vial and all samples were brought to the same volume of 500 μl with the addition of PBS.
Genomic DNA (250 ng) of each strain was transferred to Biodyne® N+ membranes (Pall Gelman Laboratory).
For reverse transcription, 100 ng of total RNA was transferred to the reaction with Prime Script reverse transcriptase (Takara, Kyoto, Japan) and random 6-mer primers.
Approximately 200 250 ng of each PCR product was transferred to Thermo-Fast 96-well PCR paltes (ABgene Corp).
Approximately 200 250 ng of each PCR product was transferred to ThermoFast 96-well PCR plates (ABgene Corp).
Approximately 200-250 ng of each PCR product was transferred to Thermo Fast 96 well PCR plates (ABgene Corp., Epsom, Surray, UK) and frozen at -20°C before cycle sequencing.
Approximately 200 250 ng of each PCR product was transferred to Thermo-Fast 96-well PCR plates (ABgene Corp., Epsom, Surray, UK) and frozen at 20°C, before cycle sequencing using an ABI3730 XL machine.
About 3.6 ng of the mixed adapters was transferred to each of the 24-wells of a 96-well plate and dried by placing the plate in a refrigerator for 2 5 days.
After P14, a subpopulation of the cells was transferred to medium with normal glucose level (NG) (5 mM).
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