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The functional effect of tumor-cell conditioned medium on the migration of neutrophil granulocytes (NG) was tested.
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For ligation, a constant 25 ng of vector was used, and varying amounts of insert ranging from 60 to 120 ng were tested for each size selection.
Of each sample different amounts of DNA between 2 and 100 ng were tested as template.
CT and NG were tested using Ligase Chain Reaction in 1999 whereas Polymerase Chain Reaction was used in 2001.
To investigate whether HRM assay can be performed on a small amount of DNA template, DNA amounts ranging from 1 to 100 ng (1, 5, 10, 25, 50 and 100 ng) were tested using a sample with an exon 19 p.E746_A750 deletion mutation present at 50% mutant allele frequency.
These analyses demonstrated that reliable results could also be obtained when more than 1 ng of template DNA was tested by HRM.
100 ng RNA per sample was tested in duplicates and results averaged.
First, the effect of various concentrations (range 31.6 1000 ng ml−1) of CHX was tested on cell survival.
† neg: 240 ng of patient Guthrie card was tested and was determined to be negative.
Several concentrations of genomic DNA were tested, and 50 ng was used for testing the SNAP primer pairs through PCR.
The sensitivity of the assay was tested using 0.5 40 ng pristine template DNA in the PCR reaction.
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