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Purified DNA (10 ng) was digested with HpyCH4 IV (NEB) according to the manufacturer's instruction.
Total genomic DNA (200 ng) was digested with two restriction enzymes Pst I and Mse I and ligated to adaptors.
Total DNA (50,000 ng) was digested with Sau3I and separated on a 2% agarose gel.
In brief, each DNA extract (500 ng) was digested with restriction enzyme ApeKI for 2 hours.
Genomic DNA (250 ng) was digested with two restriction enzymes PstI and MseI in a volume of 17.5 μl.
Briefly, DNA (250 ng) was digested with NspI (NEB, MA) and then ligated with an NspI linker supplied by Affymetrix.
Similar(31)
Thus, the PCR fragments of all samples (100 ng) were digested in a final 20 μL volume with the selected restriction endonuclease (1 U/ng DNA), according to the manufacturer's instructions (Fermentas), incubated for 3 h at 37 °C.
The unmethylated and methylated plasmids (200 ng) were digested with 20 U of either HpaII or MspI at 37°C overnight to confirm the methylation status.
Briefly, samples and control DNAs (2500 ng) were digested with the enzymes AluI and RsaI.
Plasmids (50 ng) were digested with BamHI and electrophoresed in a 1% agarose gel.
Genomic DNA samples (50 100 ng) were digested to completion with EcoRI and MseI at 37 °C in a final volume of 25 µL.
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