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We acquire values for air pressure, temperature, and humidity, and we calculate the group refractive index of air (GRA), ng, using the following equation [21].
From 10 ml of sample C, DNA yields were comparable for PowerBiofilm and QuickGene at 100 ng and 130 ng, respectively, however DNA yield was increased by almost ten-fold (870 ng) using the Maxwell platform.
The results shows (a) that the minimum amount of DNA from blood of highly parasitaemic mice that was detectable by PCR was 0.001 ng, using the ESAG6/7 and TBR1/2 primer, (b) using TBR1/2 primer for parasites purified could detect 0.000001 ng and (c) in the prepatent period PCR detect the presence of parasites earlier than parasitological techniques.
The first PCR was performed on patient P18 DNA (50 ng), using the following primers: Mrkl-seq9-F: 5'-ACTCCTGTGGTGGCACTTAGTT-3' and Mrkl-seq-9R: 5'-TCAGAGGGATGTTGCCATAAC-3'.
cDNAs were reverse transcribed from total RNA samples (100 ng) using the High Capacity cDNA Reverse Transcription Kit (LifeTechnologies-Applied Biosystems, Foster City, CA, USA).
Suzich, J.A., et al. [34] demonstrated that assembled COPV-VLP resulted in complete protection at a 50 ng dose level, and partial protection at 0.125 ng using the intra-dermal route in beagles.
Similar(41)
SD of ATR in a natural hydrophilic carrier, NG, prepared using the solvent evaporation method had significantly improved dissolution rates (P < 0.05).
RNA (0 1 ng, extracted using Ultraspec™) or DNA/RNA (0 1 ng, isolated using the Boom method) from HT-29 cells was added to RNA (5 μg) or DNA/RNA (5 μg) extracted from the neuroblastoma cell line SK-N-SH.
Labeled cRNA was prepared from 500 ng RNA using the Illumina® RNA Amplification Kit from Ambion (Austin, TX, USA).
Sodium bisulfite modification was performed on 500 ng DNA using the EZ DNA Methylation-Gold Kit (Zymo Research).
The Ct values were measured and converted to ng gDNA using the corresponding standard curves: 10 ∧ (Ct value - intercept)/slope [ng gDNA].
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