Sentence examples for ng samples of from inspiring English sources

Before conducting reverse transcription, genomic DNA contamination was quantified by amplifying 20 ng samples of raw RNA.

Replicate 150 ng samples of total RNA derived from two separate wells per time point were labelled using the Affymetrix whole transcript labelling protocol and hybridized for 16 h at 45°C to Affymetrix mouse exon 1.0 ST arrays.

The 100 ng samples of the amplicons, the negative controls of the wheat histone EST and H2O as a blank control were mixed with an equal volume of NaOH 0.6 N for alkali denaturation.

Using conditions recommended by Illumina, 200 ng samples of genomic DNA (with or without pre-processing as necessary for each experiment) were hybridised to human370CNV Infinium HD BeadChips (Illumina INC, San Diego, CA, USA).

We performed STRT RNA sequencing on 10 ng samples of total RNA from three different sample types: i) epidermal tissue (split-thickness skin grafts), ii) cultured primary KCs, and iii) HaCaT cell line.

A 400 ng sample of total RNA from each pooled sample was used for labeling.

A 200 ng sample of pET22b-MC31 plandid and a 100 ng sample of the pBCPB+ plasmid were co-transformed into Top10 bacteria, which were allowed to recover for 1 h, then spread on agar plates containing 25 µg/ml chloramphenicol, 50 µg/ml ampicillin, and 50 µg/ml 5-bromo-4-chloro-3-indolyl b-Dgalactoside (X-Gal), and grown at 37°C.

A 200 ng sample of DNA was used for the bisulfite reaction.

A 50 150 ng sample of genomic DNA or cDNA were used for PCR reactions.

To analyse the data, the relative quantification model described by Pfaffl, 2001 was used with 20 ng sample of LP-1 as calibrator.

A 4 ng sample of cDNA was analysed by quantitative PCR using pre-developed assay primers and probes for DUSP6/ MKP- 3 (Mm00650255_g1; Applied Biosystems).