Sentence examples for ng on a from inspiring English sources

Briefly, five micrograms of DNA was fragmented by sonication to 400 800 bp in size and confirmed by running 100 ng on a 2% agarose gel.

The quality of the DNA was determined by loading 200 ng on a 1% agarose gel to inspect for any degradation.

The DNA was checked for concentration and purity by reading the absorbance at 260 and 280 nM and was further tested for intactness by running 100 ng on a 1% agarose gel.

Quantitative Real Time-PCR (qRT-PCR) was performed with 1 10 diluted cDNA (20 ng) on a Mx3005P QPCR System (Stratagene) with either a SYBR Green based PCR Master Mix (Applied Biosystems / Life Technologies) or a qPCR Core kit for SYBR Green (Eurogentec).

Next, we compensate for the influence of ng on LΛair.

To determine accuracy, we performed standard global RNA-seq analysis on 500 ng of a bulk lymphocyte RNA sample and calculated RPKM values from 2.9 × 10 mapped reads.

The LOD for TCPy in meconium was 0.2 ng based on a sample weighing 0.5 g.

In figures, detection rates and NNTScope are presented relative to the amount of haemoglobin (ng ml−1) on a continuous scale.

Real-time PCR was performed with 200 ng cDNA on a 7300 Real-Time PCR System (Applied Biosystems).

PCR reactions (three biological replicates in duplicates) were performed with 0.5 ng cDNA on a Stratagene MX3000P thermocycler (qPCR MxPro v4.01) using the Absolute qPCR SYBR green ROX mix (ABgene).

DNA concentration was estimated using a fluorimeter (Hoefer DyNA Quant 200) and DNA quality was checked by migration of 150 ng DNA on a 0.6 % TAE agarose gel after electrophoretic separation for 30 minutes at 8 V per cm.