Sentence examples for ng of mouse from inspiring English sources

Stepwise insulin concentrations from 0 to 400 ng were mixed and preincubated with 30 ng of mouse anti-insulin antibody (Sigma, I2018) in 100 μL of PBST PBSS with 0.05% tween-20) in a 1.5-mL tube for 1 h.

Briefly, 50 ng of mouse IL-6 (R&D Systems, Lille, France) was adsorbed on microtitration plates overnight.

ELISA plate wells were coated with 200 ng of mouse monoclonal antibody to PR3 (clone 1B10, IgG2a; HyTest).

The efficiency of each assay was verified with a standard curve starting from 100 ng of mouse DNA, using four serial dilutions from 1 1 to 1 8.

cDNAs were synthesized starting with 300 ng of sample RNAs or 1000 ng of Mouse Universal RNA (MUR, Stratagene, La Jolla, CA) using oligo dT) primer according to kit directions.

100 ng of mouse genomic DNA, isolated from the intestinal epithelium of 3 month old C57BL/6 J-ApcMin/J mice (Jackson Laboratories), was bisulfite converted using the Epitect bisulfite kit (Qiagen).

Canonical LXR target genes, including ATP binding cassette protein A1 (anda1) and SREBP-1c (Srebf1), were up-regulated in the NG of mice treated with LXR agonists.

Some mice were injected intraperitoneally (i.p) with 500 ng of recombinant mouse IL-6 (R&D Systems).

Adult (3 4 months) wild-type and miR-146a−/− mice (on a C57/BL6 background) were injected with 100 μL of PBS or 125 ng of recombinant mouse IL-1β (in PBS) by intravascular injection.

To evaluate the concentration of secreted Cntns when overexpressed by HEK293, we loaded increasing amounts (0.5 to 25 ng) of commercialized mouse recombinant Cntn6 (R&D Systems, ref. 5890-CN) on gels and performed a Western blot analysis.

For detection of IgA antibodies in serum, total IgA was detected with 50 ng of biotinylated mouse anti-human total IgA (clone GA112, Zymed Life Technologies, Carlsbad, CA), followed by SAPE.