Sentence examples for ng of initial from inspiring English sources

Different doses of VEGF were subsequently immobilized onto the heparin-grafted FDM (hep-FDM); 19.6 ± 0.6, 39.2 ± 3.2, and 54.8 ± 8.9 ng of VEGF were tethered using 100, 300, and 500 ng of initial VEGF, respectively.

Briefly, 10 μL reaction mixtures were prepared containing 1 μL of cDNA (equivalent to 2.5 ng of initial RNA), forward and reverse primers (500 nM each) for Bin1 (pan isoforms), Bin1 (isoform 8), Dnm2, Esr1, Esr2, Pik3c2b or Gapdh (used as the housekeeping gene) (Supplementary Table 4), the kit Master mix and RNAse-free water.

cDNA corresponding to approximately 5 ng of initial RNA was next used for each quadruplicate quantitative PCR reaction.

5 µl of the first-strand cDNA reaction (corresponding to 25 ng of initial RNA used for cDNA synthesis) was added to 20 µl of the qRT-PCR mix [12.5 µl of TaqMan® Universal PCR Master Mix (Roche Molecular Systems, CA, USA), 2.25 µl of 10 µM Forward and Reverse primers each, 0.0625 µl of 10 µM probe and 2.93 µl of water].

C-DNA corresponding to 12 ng of initial total RNA served as template.

C-DNA corresponding to 12 ng of initial total RNA served as template for amplification reactions.

cDNA corresponding to 20 ng of the initial total RNA was used to perform fluorescence-based real-time PCR quantification using the LightCycler 480 Realtime PCR apparatus (Roche Diagnostics).

Real-time quantitative polymerase chain reaction experiments were performed with 1 ng of endosperm mRNA as an initial template.

Therefore, we used a sufficiently high amount (10 ng) of total RNA in the initial reverse transcription and first 10 cycles of PCR.

Obtained cDNAs were diluted in diethylpyrocarbonate (DEPC -treated water to a final concentration of 5 ng initial total RNA/μl.

Each PCR contained 30 ng of the pPhaCAB plasmid as the initial template, 250 ng of each primer, 200 μM of each dNTP, and 5 U of Taq DNA polymerase in Taq DNA polymerase reaction buffer.