Sentence examples for ng of dried from inspiring English sources
A 2 ng of dried genomic DNA in 384-well plates (Applied Biosystems) was amplified in a reaction volume of 5 μl containing 1× PCR Buffer, 1.625 mM MgCl2, 500 μM dNTPs, 100 nM each PCR primer, 0.5 U PCR enzyme (Sequenom).
We measured the level of Cry1Ab toxin in the maize kernels, resulting in an average of 67 (+/− 27) ng toxin per gram of dried tissue.
Total RNA was isolated using a modified procedure of the QIAGEN RNeasy® 96 protocol for isolation of total RNA from animal cells using spin technology (QIAGEN Inc., Valencia, CA), and a total of 500 ng total RNA dried down and stored at -20°C.
NG: Of course, it meant breaking their own rules!
Briefly, 5 ng of genomic DNA was dried down in a 384-well plate and resuspended in 10 µL of either the telomere (T) or 36B4 (S; single copy gene) PCR reaction mixture.
For use of fluorescent-labelled primers, 45 ng of template DNA is dried in the wells of 96 well polypropylene plates.
The amount of volume required for each 5 μl reaction was as follows, (10 ng of DNA needs to be dried before adding the other reagents), TaqMan Universal PCR Master Mix; 2.5 μl, TaqMan SNP Genotyping Assay; 0.25 μl and 2.25 μl of DEPC Treated Water (Quality Biological, INC , USA.
The values were expressed as ng of p-Akt per gram of dry heart weight.
The method achieved a lowest limit of detection of 0.22 ng g−1 of dry weight and a limit of quantification of 0.74 ng g−1 dry weight (zeatin riboside), and was able to recover, detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato tubers.
Sylvana varied from 24 ng g−1 dry weight (DW) at harvest to less than 1 ng g−1 DW at the end of 12 weeks storage corresponding to first indication of sprouting.
