Sentence examples for ng of active from inspiring English sources

Twenty ng of active hrMMP-2 was used and the inhibitory activity of 100 ng of mrTIMP-1 tested for 1 h.

Activity of 1U was defined as production of 1 µmole of 4-MU/min at 37°C and pH 4. Activity was converted to and reported as ng of active enzyme per ml, based on a standard reference preparation of purified rhGALNS with specific activity of 2 U/mg.

Then, the samples were incubated in 10 μl kinase assay buffer containing 20 ng of active, recombinant full-length p38 (SignalChem), and 250 μM ATP at 30°C for 25 min.

Ten ng of active C2 (determined by active site titration with E-64) [ 27] diluted in 75 μl of 0.1% (v/v) Brij-35 was mixed with 75 μl of the different IgG solutions diluted in C2 assay buffer (100 mM Bis-Tris buffer, pH 6.4, 4 mM Na2EDTA and 0.02% (w/v) NaN3) without dithiothreitol (DTT).

GST-purified fusion proteins were incubated or not with 10 ng of active PKC for 30 min at 37 °C, in the presence of 10 mM Tris-HCl (pH 7.4), 10 mM MgCl2, 0.5 mM CaCl2 and 10  μM ATP ([ γ-P]ATP 2  μCi per tube; 3000 Ci/mmol; Perkin-Elmer, Waltham, MA, USA).

Both of AU (CDK activity unit) and EU CDKK expression unit) were defined as the equivalent expression and activity of 1 ng of recombinant active CDK molecule, respectively.

PHB synthetic peptides (2 µg) (Pep I - Biotin-105RIFTSIGEDYDERVLPSIT123-NH2 and Pep II - Biotin-250QLSRSRNITYLPAGQSVLL268-NH2, atidated at C-terminus and biotinylated at N-terminus) were incubated with 20 ng of recombinant active insulin receptor in kinase buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 50 µM ATP) for 20 min at 30°C.

We do not know whether this occurs because the low protein load of 0.88 2.2 ng (200 500 MU) of active neurotoxin is not detected by the human immune system after intramuscular injections or because of the lack of complexing proteins.

125 ng and 200 pg of active IGF-I and TGF-β, respectively, as measured by ELISA, were released over 15 days.

By consensus, the following groups of patients with MDS should be regarded as candidates for iron chelating therapy: Patients with frank iron overload (e.g. stable/increasing serum ferritin > 2000 ng mL−1 without signs of active inflammation or liver disease) who are transfusion-dependent (at any frequency) and have a life expectancy of more than two years.

Addition of 10 μg/ml of CL82198 together with 5 ng active of MMP13 protein [ 17] blocked more than 90% of MMP13 activity in vitro.