Sentence examples for ng from each from inspiring English sources
One hundred ng from each of the four schizophrenic mRNA pool was hybridized together with 100 ng mRNA from the control pool on slides printed with 29 750 human cDNA clones [ 16].
Genomic DNA (100 ng) from each transgenic line was used as template.
Total RNA (∼50 ng) from each biological replicate was reverse transcribed and labeled using the Affymetrix 2-cycles labeling kit according to the manufacturer's instructions.
Total RNA (500 ng) from each sample was amplified and labelled by in vitro transcription according to the manufacturer's instructions (Illumina TotalPrep RNA amplification kit, Ambion, Texas, USA).
Total RNA (800 ng) from each target organ was subjected to reverse transcription, second strand synthesis and 1-round amplification by in vitro transcription, from which 20 µg amino allyl aRNA was labeled with Alexa Fluor 647 reactive dyes (Invitrogen).
For quantification of HIF binding sites, ChIP DNA 55 ng) from each of input, preimmune sera, PM14 and PM9 was subject to RT-Q-PCR using oligonucleotides designed to amplify putative HRE consensus sequences (Supplementary Figure S1).
500 ng RNA from each plant was reverse transcribed with 50 μM oligo dT) and SuperScript III reverse transcriptase (Invitrogen) according to the manufacturer's instructions.
Biotinylated cRNA was prepared using the Illumina RNA Amplification Kit (Ambion) according to the manufacturer's instructions starting with from 200 ng total RNA from each sample.
Five 500 ng total RNA from each donor was reverse transcribed using Omniscript (Qiagen Ltd ., and cDNA representing 2.5 ng total RNA was used in each PCR reaction.
200 ng total RNA from each sample was reversely transcribed into cDNA with SuperScript™III (Invitrogen) according to the supplier's recommendation.
The microarray hybridization procedure was carried out using 500 ng total RNA from each sample and the Agilent two color low RNA Input Linear Amplification kit (Agilent Technologies, Waldbronn, Germany).
