Hybridization solution was prepared with 825 ng each of Cy3- and Cy5-labeled cRNA (Table 2) preparations using the In situ Hybridization Plus kit (Agilent Technologies).
The PCR products were purified and quantified as described in Hoshino et al. (Hoshino and Morimoto [2008]), and 100 ng each of the DNA samples were loaded on DGGE based on the method of Muyzer et al. (Muyzer et al. [1993]) using the D Code System (Bio-Rad, Hercules, CA).
To activate the Akt pathway, cells were transfected with 100 ng each of PI3-K p110α and Akt expression vectors.
Hybridization solution was prepared with 825 ng each of Cy3- and Cy5-labeled cRNA preparations using the In situ hybridization kit plus (Agilent Technologies, Santa Clara, CA, USA).
BN and LLC-PK1 cells were cotransfected with 300 ng of −800 meg-Luc and 300 ng each of either PPARα or PPARγ cDNA.
About 100 ng each of the converted DNA was amplified with primers designed for a consensus IAP LTR (GenBank accession no. M17551) [22] and the skeletal α-actin promoter (accession no. M12347).
800 ng each of the pGL4.15cmv[5' UTR] plasmids and control pGL4.80cmv plasmid was co-transfected into each well of a 24 well plate containing sub-confluent SH-SY5Y cells.
In this assay, 100 ng each of the P1 and P2 Fab were used as antigen in ELISA wells, and anti-gp120 mAbs were tested for reactivity with P1 and P2.
PCR reactions for the mutant strand synthesis reaction contained 25 ng of dTip60 template DNA, 125 ng each of forward and reverse primer, and PfuTurbo DNA polymerase (Stratagene, La Jolla, CA, USA).
825 ng each of cyanine 3 and cyanine5 labeled cRNA were used for each array.
175 ng each of the pyrotagged summer and winter 16S rRNA gene amplicons and 50 ng each of the pyrotagged summer and winter 18S rRNA gene amplicons were pyrosequenced on an eight-lane Roche picotiter plate.
