Sentence examples for ng data not from inspiring English sources

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However, the expression of the DHCDH of P. chrysosporium was recently reported [ 81]. > -wrap-foot> NG data not given aHomologous overexpression bValue refers to cell-free extract after cell disruption and not to cultivation volume The advantages of recombinant protein expression are a fast, reliable and efficient enzyme production and the possibility to generate genetically engineered enzymes.

Traditional PCR method based on gel electrophoresis analysis gave linear standard curve in the concentration range from 10−4 to 10−1 ng DNA (equivalent to 5.2 × 102 to 5.2 × 105 cells) (Figures 2 and 3), but showed apparent saturation at higher amounts of DNA (i.e., 1 and 10 ng, data not shown).

This effect was lost when higher amounts of CRTR-1 plasmid were used (such as 150 ng and 200 ng; data not shown).

A more quantitative measurement was performed on the material eluted from spot number 6 by quantitative amino acid analysis which indicated a total peptides concentration of 180 ng (data not shown).

When assayed by FACS, we find that human B cells bind both hu and mu BLyS, tested at the optimal concentration used in our survival assays (100 ng) (Figure 3A) and also at lower concentrations (1 and 10 ng, data not shown).

P2X1, P2X2 and P2X5 mRNAs were not detected despite increasing the amount of starting mRNA template to 10 ng (data not shown).

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The basal levels of IGF-I and -II produced by both the MCF-10A and the Hs578T cells, as measured by radioimmunoassays previously described (Taylor et al, 1990; Davies et al, 1991) were found to be lower than the detection limits of the assays and hence negligible in relation to the doses required for IGF responses in these cells (i.e. <1 ng ml−1; data not shown).

We also observed that as the amount of DNA decreased, the reproducibility of the reaction was lower, giving a higher inter-tube and inter-assay variation, mainly in the 1 30 (2 ng) and 1 40 (1.5 ng) dilutions (data not shown).

To generate a reference, increasing concentrations of pure AFB1 were dissolved in 70% MeOH and the number of Salmonella CFUs that reverted from his − to his + increased in a concentration-dependent manner from 0 ng g−1 (70% MeOH only) to 10,000 ng g−1 (data not shown).

The presence of the IGFBP-3 antisense mRNA reduced this to 327 ng ml−1, although there was also some suppressive effect of the sense mRNA with IGFBP-3 levels at 527 ng ml−1 (data not shown) and this appeared to correspond to a small increase in cell death with the sense mRNA.

Absolute areas (without internal standard addition) for all target compounds were compared after analysis of a mix of compounds at 500 ng L−1 (data not shown).

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